||Houot L, Floutier M, Marteyn B, Michaut M, Picciocchi A, Legrain P, Aude JC, Cassier-Chauvat C, Chauvat F.
||Cadmium triggers an integrated reprogramming of the metabolism of Synechocystis PCC6803, under the control of the Slr1738 regulator
||BMC Genomics. 2007 Oct 2;8:350.
||300 ml of mid-log phase liquid cultures (2.5 × 107cells.ml-1) grown in standard conditions were rapidly concentrated 40-fold by centrifugation and spotted as 20 μl dots on BG11 plates with, or without (control samples) H2O2 (3 mM). Then, plates were incubated for the indicated times (in min) prior to cell harvest and fast disruption with an Eaton press.
||The unicellular cyanobacterium Synechocystis PCC6803 was grown at 30°C in liquid BG11 medium enriched with Na2CO3 (3.78 mM, final concentration), under continuous white light of standard fluence (2,500 luxes, i.e. 31.25 μE.m-2.s-1). Growth control condition were the same as in treatment without the addtion of H2O2 (3 mM).
||Cadmium/oxidative stress/Zn excess/Iron limitation/Iron excess