||Koskinen S, Hakkila K, Gunnelius L, Kurkela J, Wada H, Tyystjärvi T.
||In vivo recruitment analysis and a mutant strain without any group 2 σ factor reveal roles of different σ factors in cyanobacteria.
||Mol Microbiol. 2015 Sep 10
||The OD 730 of cell culture was set to 0.1, and the cells were grown in standard growth conditions for 3 days. Cultures (OD730 = 1, 40 ml) were rapidly cooled by pouring them into two frozen centrifuge tubes, and cells were harvested by centrifugation at +4°C. RNA isolation
||The cells of the glucose-tolerant strain of Synechocystis sp. PCC 6803 were grown in BG-11 medium supplemented with 20 mM HEPES, pH 7.5, at 32°C, under continuous illumina- tion at photosynthetic photon flux density (PPFD) of 40 μmol m−2 s−1 in ambient air. These growth conditions are referred to as standard conditions. Liquid cultures (30 ml) were grown in 100 ml Erlenmayer flasks that were shaken at 90 r.p.m
||Normal condition(mutant vs wild type)