||Paithoonrangsarid K, Shoumskaya MA, Kanesaki Y, Satoh S, Tabata S, Los DA, Zinchenko VV, Hayashi H, Tanticharoen M, Suzuki I, Murata N.
||Five histidine kinases perceive osmotic stress and regulate distinct sets of genes in Synechocystis.
||J Biol Chem. 2004 Dec 17;279(51):53078-86
||For exposure of cells to hyperosmotic stress, cells in a 50-ml culture were grown under standard conditions for 16 h (when the optical density at 730 nm of the culture was 0.3), and then 5.0 m sorbitol was added to the culture to give a final concentration at 0.5 m. The duration of incubation under osmotic stress was 20 min.
||Synechocystis PCC 6803 GT cells were grown photoautotrophically at 34 °C in BG-11 medium buffered with 20 mm HEPES-NaOH (pH 7.5) under continuous illumination from incandescent lamps at 70 μmol of photons/m2/s, with aeration by air that contained 1% CO2.